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| 引用本文: | 丁效东,张士荣. NaCl对大麦硝态氮吸收动力学特征的影响[J]. 中国生态农业学报(中英文), 2015, 23(11): 1423-1428 |
| DING Xiaodong,ZHANG Shirong. Kinetics characteristics of NO3- absorption of barley (Hordeum vulgare L.) pretreated with different concentrations of NaCl and NO3-N[J]. Chinese Journal of Eco-Agriculture, 2015, 23(11): 1423-1428 |
| DOI： | 10.13930/j.cnki.cjea.141346 |
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| NaCl对大麦硝态氮吸收动力学特征的影响 |
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丁效东, 张士荣
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青岛农业大学资源与环境学院 青岛 266109
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| 摘要: 采用营养液培养方法研究了不同盐分和氮水平对大麦‘鉴四’(Hordeum vulgare L.)生长及硝态氮(NO3--N)吸收动力学参数特征的影响。结果表明: 不同浓度NO3--N与NaCl预处理后, 大麦对NO3-吸收符合离子吸收动力学模型, 其吸收动力学参数表现为NO3-预处理浓度增加后, Vmax增大, Km值增加, 但增加的幅度不一致。对高亲和力系统来说, 所有预处理大麦的NO3--N吸收曲线均符合Michaelis-Menten方程的描述。1 mmolL-l NO3--N预处理中, 120 mmolL-l NaCl预处理比1 mmolL-l NaCl预处理显著提高了NO3--N的吸收速率; 10 mmolL1 NO3--N预处理时, 120 mmolL-l NaCl预处理与1 mmolL-l NaCl预处理无显著性差异。表明低氮环境下生长的大麦, 其根系NO3--N高亲和力系统受NaCl影响较大。对低亲和力系统来说, 所有预处理大麦的NO3--N吸收曲线均符合Michaelis-Menten方程的描述。在1 mmolL-l NO3--N预处理中, 与1 mmolL-l NaCl预处理相比, 120 mmolL1 NaCl胁迫的大麦NO3--N吸收速率显著提高; 10 mmolL-l NO3--N预处理中, 120 mmolL-l NaCl与1 mmolL-l NaCl处理相比, 降低了大麦的NO3--N吸收速率。表明低氮环境下生长的大麦, 盐胁迫对其根系低亲和力系统有促进作用, 而高氮环境下生长的大麦, 盐胁迫对其低亲和力系统的NO3--N吸收没提高作用。 |
| 关键词: 大麦 盐胁迫 NO3--N吸收 动力学特征 亲和力系统 预处理 |
| 中图分类号: |
| 基金项目:国家自然科学基金项目(31201693)、青岛农业大学高层次人才基金项目(1115036)资助 |
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| Kinetics characteristics of NO3- absorption of barley (Hordeum vulgare L.) pretreated with different concentrations of NaCl and NO3-N |
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DING Xiaodong, ZHANG Shirong
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College of Resources and Environment, Qingdao Agricultural University, Qingdao 266109, China
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| Abstract: To learn nitrogen absorption characteristics of plant under salt stress, the NO3--N absorption ability of barley (Hordeum vulgare L.) cultivar ‘Jian 4’ pretreated with NaCl and NO3--N were investigated using culture solution. The pretreatment concentrations of NaCl were 1 mmolL-1 (CK) and 120 mmolL-1, those of NO3--N were 1 mmol (NO3--N)L-1 and 10 mmol (NO3--N)L-1. Barley growth and NO3--N absorption were measured and the kinetics of NO3--N absorption of high- affinity transport system and low-affinity transport system of barley were investigated. The results showed that the uptake of NO3--N of barley pretreated with different concentrations of NaCl and NO3--N was in accordance with Michelis-Menten equation. Also the uptake kinetics parameters Vmax and Km were enhanced with increasing pretreatment concentration of NO3--N. For high-affinity system, the uptake of NO3--N of barley was in accordance with Michaelis-Menten equation for all the pretreatments. Under 1 mmol(NO3--N)L-1 pretreatment, compared with 1 mmolL-1 NaCl treatment, 120 mmolL1 NaCl pretreatment significantly increased barley uptake rate of NO3--N; while under 10 mmol(NO3--N)L-1, no significant difference in the rate of uptake of NO3--N was observed between 1 mmolL1 NaCl and 120 mmolL-1 NaCl treatments. This indicated that in low nitrogen environment, NaCl restrained uptake of NO3--N of high-affinity system. For low-affinity systems, the uptake rate of NO3--N of barley was in accordance with Michaelis-Menten equation for all pretreatments. Under 1 mmol(NO3--N)L-1 pretreatment, compared with 1 mmolL-1 NaCl treatment, 120 mmolL-1 NaCl treatment significantly increased the rate of uptake of NO3--N. With 10 mmolL-1 NO3--N pretreatment, the uptake rate of NO3--N under 120 mmolL-1 NaCl was lower than that of under 1 mmolL-1 NaCl pretreatment. This showed that under low nitrogen environment, salt stress improved root uptake of NO3--N in low-affinity system. However, under high nitrogen environment, NaCl stress did not alter obviously root uptake of NO3--N in low-affinity systems. |
| Keyword: Hordeum vulgare L. Salt stress NO3--N uptake Kinetic characteristics Affinity system Pretreatment |
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