| 摘要: 本研究通过对牛粪工艺发酵后的沼液进行代谢物质及微生物结构组成分析,旨在为充分利用沼液资源,开发高效环保的有机液肥产品提供理论支撑。采用液质联用(LC-MS)和16S rRNA基因扩增子高通量测序技术研究沼液中代谢物质和微生物群落结构组成。结果表明,沼液中代谢物以脂类和类脂分子为主,并鉴定出17种参与植物生长发育的物质。沼液中细菌群落物种丰富度指数、香农指数、辛普森指数、Chao1和ACE分别为2558,7.53,0.975,2903和2975,细菌相对丰度大于1%的菌门有变形菌门、拟杆菌门、厚壁菌门、互养菌门、螺旋体门、广古菌门、绿弯菌门、圣诞岛盐菌门、软壁菌门、放线菌门和泉古菌门,沼液中细菌功能类群主要为化能异养型和好氧化能异养型细菌,参与碳氮循环过程。综合分析表明,根据对沼液中丰富的代谢物质和微生物群落组成及相关功能分析,发现沼液作为有机肥具有应用潜能,其具体肥效有待进一步通过田间试验验证。 |
| 关键词: 沼液 代谢物质 高通量测序 细菌多样性 |
| 中图分类号:S216.4 |
| 基金项目:国家重点研发计划项目（2016YFD0201100）；河北省高等学校科学技术研究项目（ZD2019135）； |
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| Analysis of metabolites and bacterial community in fermentation biogas slurry |
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YANG Zhichang1,2, SHAO Jianzhu1,2, ZHANG Xueying1,2, LI Guofang1,2, TAN Ming1,2, SUN Jianshe1,2, LIN Meiling1,2, JIANG Kangning1,2, ZHANG He1,2
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1.College of Horticulture,Hebei Agricultural University Baoding 071000;2.China
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| Abstract: This study analyzed the metabolites and microbial structure of biogas slurry fermented by cow dung process, aiming to provide theoretical support for making full use of biogas slurry resources and developing efficient and environmentally friendly organic liquid fertilizer products. The liquid mass spectrometry (LC-MS) and 16S rRNA gene amplification high-throughput sequencing method was adopted to study metabolites and microbial community structure in biogas slurry. The results showed that lipids and lipid-like molecules were the main metabolites in biogas slurry, and 17 kinds of metabolites involved in plant growth and development were identified. The species richness index, Shannon index, Simpson index, Chao1 and ACE index were 2558,7.53,0.975,2903 and 2975, respectively, The relative abundances of bacterial phyla than 1%, they were Proteobacteria, Bacteroidetes, Firmicutes, Synergistetes, Spirochaetes, Euryarchaeota, Chloroflexi, Kiritimatiellaeota, Tenericutes, Actinobacteria, and Crenarchaeota.And the main functional groups of bacteria in biogas slurry are chemoheterotrophy bacteria and aerobic chemoheterotrophy bacteria, which involve in the carbon and nitrogen cycles. Comprehensive analysis showed that the biogas slurry had potential application as an organic fertilizer according to the analysis of the abundant metabolite, microbial community composition and related functions, and its fertilizer efficiency were needed to be further under filed conditions to study. |
| Keyword: Biogas slurry Metabolites High-throughput sequencing Bacterial biodiversity |