| 摘要: 应用Biolog方法研究了转Bt基因棉粉碎叶腐解对土壤微生物群落结构功能多样性的影响。取腐解10 d、25 d、40 d、55 d、70 d土样分析土壤微生物群落多样性指数及土壤微生物对聚合物、胺类、氨基酸、糖、羧酸和其他类碳源利用情况。结果表明: 在腐解过程中, 转Bt基因棉粉碎叶土壤微生物群落丰富度下降, 群落多样性显著降低, 而群落优势集中性明显提高; 转Bt基因棉粉碎叶影响了土壤微生物群落对碳源的利用程度, 表现为可显著增加对糖类、胺类和氨基酸类碳源的利用, 初期显著降低对羧酸类碳源的利用, 对聚合物类和其他类碳源的利用率无显著影响; 主成分分析表明转Bt基因棉粉碎叶对土壤微生物群落原有结构功能影响具有持续性。 |
| 关键词: 转Bt基因棉 腐解 土壤微生物 群落多样性 碳源 主成分分析 |
| 中图分类号: |
| 基金项目:山西省自然科学基金项目(20051073)和山西农业大学博士科研启动基金项目资助 |
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| Effect of transgenic Bt cotton leaves on soil microbial community diversity |
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ZHANG Mei-Jun, YANG Wu-De
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College of Agronomy, Shanxi Agricultural University, Taigu 030801, China
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| Abstract: The diversity of soil microbial community and utilization pattern of polymer, amine, amino acid, carbohydrate, carboxylic acid and miscellaneous carbons were assessed via Biolog method on the 10th, 25th, 40th, 55th and 70th day of transgenic Bt cotton ground leaves decomposition. The results show decrease in soil microbial community richness and diversity, and increase in concentration index with ground leaves of transgenic Bt cotton. Ground leaves of transgenic Bt cotton has no effect on polymer and miscellaneous carbon utilization by soil microbes. It, however, obviously improves amine, amino acid and carbohydrate carbon utilization and also significantly debases carboxylic acid carbon utilization at the early stage of decomposition. Principal component analysis indicates that transgenic Bt cotton ground leaves have a sustained influence on indigenous soil microbial community structure and function. |
| Keyword: Transgenic Bt cotton, Decomposition, Soil microbe, Community diversity, Carbon resources, Principal component analysis |
